The simplified procedure is based on ourinnovative protein purification chemistry and customdesigned fast spin columns. Up to 1 mg of His taggedprotein can be purified in 5 minutes and eluted in aslittle as 100 ul of His-Elution Buffer. The purifiedprotein can be used directly for enzymatic assays,protein biochemical analyses, SDS-PAGE and otherapplications. The product has been optimized formaximal protein purity: a single protein band is visibleby Coomassie blue staining on SDS-PAGE gel. The straightforward spin wash elute protocol dramatically simplifies protein purification: get results in minutes, not hours.